MersV1, Main, Exploration, bibRecord, 002D08

MALDI analysis of oligonucleotides directly from montmorillonite.

Identifieur interne : 002D08 ( Main/Exploration ); précédent : 002D07; suivant : 002D09

MALDI analysis of oligonucleotides directly from montmorillonite.

Auteurs : Dmitri V. Zagorevskii [États-Unis] ; Michael F. Aldersley ; James P. Ferris

Source :

Journal of the American Society for Mass Spectrometry [ 1044-0305 ] ; 2006.

RBID : pubmed:16809045

Descripteurs français

KwdFr :
- Bentonite (), Oligonucléotides (), Oligonucléotides (analyse), Prélèvement biologique (), Spectrométrie de masse MALDI ().
MESH :
- analyse : Oligonucléotides.
- Bentonite, Oligonucléotides, Prélèvement biologique, Spectrométrie de masse MALDI.

English descriptors

KwdEn :
- Bentonite (chemistry), Oligonucleotides (analysis), Oligonucleotides (chemistry), Specimen Handling (methods), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods).
MESH :
- chemical , analysis : Oligonucleotides.
- chemical , chemistry : Bentonite, Oligonucleotides.
- methods : Specimen Handling, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.

Abstract

Oligonucleotides synthesized on a montmorillonite catalyst were analyzed directly. By mixing the catalyst with a matrix (2,4,6-trihydroxyacetophenone or 6-aza-2-thiothymine) and dibasic ammonium citrate, higher molecular weight products were detected compared with "classical" methods such as gel electrophoresis and HPLC with UV as a detector. The oligomers (30-mers and higher) were detected by mass spectrometry even though their concentration was less than 10(-4)% of the total content of the RNA. This method is different from the (MALDI) analysis of the eluates from montmorillonite, which otherwise requires desalting. Placing reaction mixtures with a high concentration of buffers on homoionic, preferably Li-containing, montmorillonite does not require desalting.

DOI: 10.1016/j.jasms.2006.05.012
PubMed: 16809045

Affiliations:

États-Unis

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">MALDI analysis of oligonucleotides directly from montmorillonite.</title>
<author><name sortKey="Zagorevskii, Dmitri V" sort="Zagorevskii, Dmitri V" uniqKey="Zagorevskii D" first="Dmitri V" last="Zagorevskii">Dmitri V. Zagorevskii</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute,209C Cogswell Laboratory, Troy, New York 12180, USA. zagord@rpi.edu</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute,209C Cogswell Laboratory, Troy, New York 12180</wicri:regionArea>
<wicri:noRegion>New York 12180</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Aldersley, Michael F" sort="Aldersley, Michael F" uniqKey="Aldersley M" first="Michael F" last="Aldersley">Michael F. Aldersley</name>
</author>
<author><name sortKey="Ferris, James P" sort="Ferris, James P" uniqKey="Ferris J" first="James P" last="Ferris">James P. Ferris</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2006">2006</date>
<idno type="RBID">pubmed:16809045</idno>
<idno type="pmid">16809045</idno>
<idno type="doi">10.1016/j.jasms.2006.05.012</idno>
<idno type="wicri:Area/PubMed/Corpus">002248</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002248</idno>
<idno type="wicri:Area/PubMed/Curation">002248</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002248</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002109</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002109</idno>
<idno type="wicri:Area/Ncbi/Merge">000442</idno>
<idno type="wicri:Area/Ncbi/Curation">000442</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000442</idno>
<idno type="wicri:doubleKey">1044-0305:2006:Zagorevskii D:maldi:analysis:of</idno>
<idno type="wicri:Area/Main/Merge">002D35</idno>
<idno type="wicri:Area/Main/Curation">002D08</idno>
<idno type="wicri:Area/Main/Exploration">002D08</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">MALDI analysis of oligonucleotides directly from montmorillonite.</title>
<author><name sortKey="Zagorevskii, Dmitri V" sort="Zagorevskii, Dmitri V" uniqKey="Zagorevskii D" first="Dmitri V" last="Zagorevskii">Dmitri V. Zagorevskii</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute,209C Cogswell Laboratory, Troy, New York 12180, USA. zagord@rpi.edu</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute,209C Cogswell Laboratory, Troy, New York 12180</wicri:regionArea>
<wicri:noRegion>New York 12180</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Aldersley, Michael F" sort="Aldersley, Michael F" uniqKey="Aldersley M" first="Michael F" last="Aldersley">Michael F. Aldersley</name>
</author>
<author><name sortKey="Ferris, James P" sort="Ferris, James P" uniqKey="Ferris J" first="James P" last="Ferris">James P. Ferris</name>
</author>
</analytic>
<series><title level="j">Journal of the American Society for Mass Spectrometry</title>
<idno type="ISSN">1044-0305</idno>
<imprint><date when="2006" type="published">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bentonite (chemistry)</term>
<term>Oligonucleotides (analysis)</term>
<term>Oligonucleotides (chemistry)</term>
<term>Specimen Handling (methods)</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Bentonite ()</term>
<term>Oligonucléotides ()</term>
<term>Oligonucléotides (analyse)</term>
<term>Prélèvement biologique ()</term>
<term>Spectrométrie de masse MALDI ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Oligonucleotides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Bentonite</term>
<term>Oligonucleotides</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Oligonucléotides</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Specimen Handling</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Bentonite</term>
<term>Oligonucléotides</term>
<term>Prélèvement biologique</term>
<term>Spectrométrie de masse MALDI</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Oligonucleotides synthesized on a montmorillonite catalyst were analyzed directly. By mixing the catalyst with a matrix (2,4,6-trihydroxyacetophenone or 6-aza-2-thiothymine) and dibasic ammonium citrate, higher molecular weight products were detected compared with "classical" methods such as gel electrophoresis and HPLC with UV as a detector. The oligomers (30-mers and higher) were detected by mass spectrometry even though their concentration was less than 10(-4)% of the total content of the RNA. This method is different from the (MALDI) analysis of the eluates from montmorillonite, which otherwise requires desalting. Placing reaction mixtures with a high concentration of buffers on homoionic, preferably Li-containing, montmorillonite does not require desalting.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><noCountry><name sortKey="Aldersley, Michael F" sort="Aldersley, Michael F" uniqKey="Aldersley M" first="Michael F" last="Aldersley">Michael F. Aldersley</name>
<name sortKey="Ferris, James P" sort="Ferris, James P" uniqKey="Ferris J" first="James P" last="Ferris">James P. Ferris</name>
</noCountry>
<country name="États-Unis"><noRegion><name sortKey="Zagorevskii, Dmitri V" sort="Zagorevskii, Dmitri V" uniqKey="Zagorevskii D" first="Dmitri V" last="Zagorevskii">Dmitri V. Zagorevskii</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002D08 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002D08 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:16809045
   |texte=   MALDI analysis of oligonucleotides directly from montmorillonite.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:16809045" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

Serveur d'exploration MERS

MALDI analysis of oligonucleotides directly from montmorillonite.

MALDI analysis of oligonucleotides directly from montmorillonite.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.